Analysis of HBB-HS-107 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2242-LGZ)

Introduction

Gene description: beta-globin hypersensitive site -107<br />Gene type: biological region<br />Also known as: HS-107<br />This element represents a DNase I hypersensitive site (HS) located approximately 107 kb upstream of the HBE1 (hemoglobin, epsilon 1) gene in the beta-globin gene cluster. This HS was observed in murine erythroleukemia cells carrying human chromosome 11. It is present within a large retroviral insertion (LINE:L1PA3 type) in the human genome, and therefore the mouse genome lacks an orthologous site.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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