Analysis of HEATR1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1048-LGZ)

Introduction

Official Full Name: HEAT repeat containing 1<br />Also known as: BAP28; UTP10<br />Enables RNA binding activity. Involved in positive regulation of rRNA processing and positive regulation of transcription by RNA polymerase I. Located in fibrillar center and mitochondrion. Implicated in pancreatic ductal carcinoma. Biomarker of glioblastoma.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Bulbus Fritillariae Cirrhosae by Real-Time PCR Method (CAT#: STEM-MB-4011-LGZ)
Analysis of PRAMEF18 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1379-LGZ)
Analysis of Neospora Spp. by Real-Time PCR Method (CAT#: STEM-MB-3588-LGZ)
Analysis of Dermatophilus Congolensis by Real-Time PCR Method (CAT#: STEM-MB-2585-LGZ)
Analysis of Microsporum Nanum by Real-Time PCR Method (CAT#: STEM-MB-3264-LGZ)
Analysis of ATP13A2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0083-LGZ)
Analysis of LYST Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0455-LGZ)
Analysis of Streptococcus Canis by Real-Time PCR Method (CAT#: STEM-MB-3119-LGZ)