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Analysis of Hepcidin (Human) by ELISA (CAT#: STEM-MB-0890-LGZ)

Introduction

Hepcidin (Hepc) is a regulator of iron metabolism. Hepcidin inhibits the transport of iron ions by binding to ferroportin (FPN) located on the basolateral surface of intestinal enterocytes and the plasma membrane of reticuloendothelial cells (macrophages). Hepc eventually decomposes the transporter in the lysosome, inhibits FPN and prevents the export of iron ions, which are sequestered in the cell.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Growth Factors, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids
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