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Analysis of Heregulinβ-1 / NRG1 / HRG / HGL by ELISA (CAT#: STEM-MB-1560-LGZ)

Introduction

Neuregulin 1 (NRG1) was originally identified as a 44 kD glycoprotein that interacts with NEU/ERBB2 receptor tyrosine kinase to increase the phosphorylation of tyrosine residues. A variety of different isomers are produced from NRG1 gene by alternative splicing. These subtypes include heregulins (HRGs), nerve growth factors (GGFS), and sensory and motor neuron derived factors (SMDF). Neuregulin and its various subtypes are derived from a family of four structurally related proteins that are part of the EGF protein family. These proteins play a variety of roles not only in the development of the nervous system but also in vertebrate embryonic development, including heart development, neurembrane and oligodendrocyte differentiation, some aspects of neural development, and the formation of neuromuscular synapses.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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