Analysis of HES5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1326-LGZ)

Introduction

Official Full Name: hes family bHLH transcription factor 5<br />Also known as: bHLHb38<br />This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of OR52R1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2358-LGZ)
Analysis of Microsporum Canis by Real-Time PCR Method (CAT#: STEM-MB-3256-LGZ)
Analysis of SHISA4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0659-LGZ)
Analysis of SIPA1L2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0658-LGZ)
Analysis of Eperythrozoon Lirei by Real-Time PCR Method (CAT#: STEM-MB-3920-LGZ)
Analysis of S100A5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0331-LGZ)
Analysis of DUSP10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0910-LGZ)
Analysis of TRIM68 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2177-LGZ)