Analysis of Histone H3 (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0255-LGZ)

Introduction

Histone H3 (21-44), derived from amino acids 21-44 of histone H 3, is commonly used as a substrate for methylation detection (such as protein arginine methyltransferase).

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Principle Applications Procedure Materials Notes Related Products

Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Rabbit Polyclonal
Detection Antibody: Rabbit Polyclonal

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Whole Cell Lysate

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