Analysis of HSP27/HspB1 (Human) by ELISA (CAT#: STEM-MB-0894-LGZ)

Introduction

Heat shock protein 27(HSP-27) is a member of the low molecular weight heat shock protein family. Hsp27 is an important protein involved in drug resistance, cell growth, apoptosis, tumor occurrence and metastasis, etc. These functions mediated by Hsp27 may be related to its effects on other proteins. When the cells were not stressed, the expression of Hsp27 was low and mainly presented in large polymers, and Hsp27 under such polymers generally had no activity. When cells are stressed, Hsp27 expression is increased and phosphorylated, making Hsp27 active and performing some of its functions.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Muscle Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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