Analysis of Human T-cell Lymphoma Virus (HTLV) by Real-Time PCR Method (CAT#: STEM-MB-3873-LGZ)

Introduction

Human T Cell lymphotropic viruses (HTLV) are a family of retroviruses. Currently, four types of this virus have been identified which are known to infect humans with a pathological consequence: HTLV-1, HTLV-2, HTLV-3, and HTLV-4. These viruses are known for their oncogenic potential as well as etiology in other disease processes.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.