Analysis of HumanT-lymphotropicVirus2(HTLV-2) by Real-Time PCR Method (CAT#: STEM-MB-2555-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.

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Principle Applications Procedure Materials Notes Related Products

Principle

HumanT-lymphotropicVirus2 (HTLV-2) is an RNA virus that can cause blood cancer or other metastatic cancers after infection. HTLV-2 and HTLV-1 have 70% homology and are also distantly related to HIV (HIV-I). The virus mainly infects CD4+T lymphocytes in the body and can cause adult T lymphocyte leukemia/lymphoma and other diseases, mainly through blood transfusion, needle, sexual behavior, mother-to-child transmission, posing a threat to human health. Therefore, rapid and sensitive detection of HTLV virus is of great significance. Based on RT-PCR technology, it has high specificity. The primers are designed according to the highly conserved region of HTLV virus type 2, which will not cross-react with RNA of other viruses.

Applications

Specifically testing for human T-lymphotropic virus type 2, only for scientific purposes.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: Sample RNA

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