Analysis of ICAM-1 (Human) by ELISA (CAT#: STEM-MB-0899-LGZ)

Introduction

Intercellular Cell Adhesion Molecule-1 (ICAM-1) is a transmembrane protein with an amino-terminal extracellular domain, a single transmembrane domain, and a carboxy-terminal cytoplasmic domain. ICAM-1 plays an important role in promoting the adhesion of inflammatory sites, controlling tumor progression and metastasis, and regulating the body's immune response. Through specific binding to its receptors, it enhances the adhesion between leukocytes, inflammatory cells and tumor cells and endothelial cells, promotes the activation of endothelial cells, and makes it easier for them to penetrate the endothelium.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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