Analysis of IFITM5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2268-LGZ)

Introduction

Official Full Name: interferon induced transmembrane protein 5
Also known as: OI5; BRIL; DSPA1; Hrmp1; fragilis4
This gene encodes a membrane protein that plays a role in bone mineralization. This gene is located on chromosome 11 of the interferon-induced related gene cluster, however, this gene has not been shown to be interferon-inducible. A similar gene, located in a gene cluster on mouse chromosome 7, is a member of the interferon-induced fragility gene family. This mouse gene encodes a transmembrane protein described as involved in germ cell competence. Mutations in the 5'UTR of this gene are associated with osteogenesis imperfecta type V.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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