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Analysis of IGF-1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0276-LGZ)

Introduction

Insulin-like growth factor-1 (IGF-1) is a hormone that, along with growth hormone (GH), helps promote normal bone and tissue growth and development. IGF-1 testing can be used to evaluate adolescents and children with growth retardation. IGF-1 is primarily produced by the liver, skeletal muscle, and many other tissues in response to GH stimulation. IGF-1 mediates many of the effects of GH, stimulating the growth of bone and other tissues, and promoting the production of muscle mass. IGF-1 also plays a role in non-growth activities such as glucose, fat metabolism and is associated with metabolic syndrome.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum
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