Analysis of IGF-1R (Human) by ELISA (CAT#: STEM-MB-0915-LGZ)

Introduction

IGF-I R (Insulin-like Growth Factor I Receptor) is a receptor for insulin-like growth factor 1, belongs to receptor tyrosine kinases (receptor tyrosine kinases, RTKs), and is a member of the insulin receptor subfamily. IGF-l R is a kind of transmembrane receptor, the extracellular α subunit determines the specificity of ligand binding, and the transmembrane β subunit passes through the cell membrane and conducts ligand-induced signals. IGF-lR can be activated by insulin-like growth factor (IGF-I or IGF-II), causing phosphorylation of its own tyrosine kinase domain and initiating intracellular signal transduction to regulate cell growth and differentiation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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