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Analysis of IgG1 (Mouse) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0284-LGZ)

Introduction

Immunoglobulin G (IgG) is a type of antibody produced and released by plasma B cells. IgG is the main type of antibody in blood and extracellular fluid, which can control the infection of human tissue. Antibodies protect the body from infection by binding to many pathogens, such as viruses, bacteria and fungi. In humans, IgG has four subtypes IgG1, IgG2, IgG3 and IgG4. In mice and rats, IgG also has four subtypes—IgG1, IgG2a, IgG2b, and IgG3.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Assay Development

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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