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Analysis of IL-12p70 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0297-LGZ)

Introduction

Interleukin-12 (IL-12) is a cytokine with a wide range of biological activities, mainly produced by activated inflammatory cells. IL-12 is a heterodimer composed of two chains of p35 and p40 (IL-12β), and the two subunits are linked together by disulfide bonds. This structure is rare in the discovered IL. Typically, the amount of p40 far exceeds that of p70.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Mouse Monoclonal
Recombinant Standards: Expressed in Insect cell line

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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