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Analysis of IL-27 (Human) by ELISA (CAT#: STEM-MB-0973-LGZ)

Introduction

IL-27 is a pleiotropic cytokine with distinct immunomodulatory activities under physiological and pathological conditions. IL-27 enhances the function of Th1 and CD8+ T cells, promotes the development of Tfh and Tr1, and inhibits the function of Th2, Treg, Th9 and Th17 cells. IL-27 is also involved in regulating the immune response of B cells, NK cells, DCs and macrophages. IL-27 production is tightly regulated at both transcriptional and post-transcriptional levels. Given its wide-ranging effects on immune regulation, IL-27 has been implicated in the pathogenesis of autoimmune and infectious diseases, as well as cancer.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids