Analysis of IL-6 (Human) by ELISA (CAT#: STEM-MB-0992-LGZ)

Introduction

Interleukin-6 is a cytokine of the chemokine family. IL-6 is synthesized by a variety of cells, including activated T and B cells, monocyte-macrophages, endothelial cells, epithelial cells, and fibroblasts. There are many target cells that IL-6 acts on, including macrophages, liver cells, resting T cells, activated B cells, and plasma cells; its biological effects are also very complex, and it was once called B cell stimulating factor 2 (bsf-2 ), 26KD protein, B cell differentiation factor (bCDf), hepatocyte stimulating factor (hsf), etc.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

Other recommended products

Analysis of IL-7 (Non-human primate) by ELISA (CAT#: STEM-MB-0998-LGZ)
Analysis of Human SP-D(Pulmonary surfactant-associated protein D) by ELISA (CAT#: STEM-MB-1765-LGZ)
Analysis of Rat PTHRP(Parathyroid Hormone Related Protein) by ELISA (CAT#: STEM-MB-2070-LGZ)
Analysis of CTLA-4 (Mouse) by ELISA (CAT#: STEM-MB-0763-LGZ)
Analysis of IL-6R (Human) by ELISA (CAT#: STEM-MB-0996-LGZ)
Analysis of Plant NADH Dehydrogenase (NADHD) by ELISA (CAT#: STEM-MB-2358-LGZ)
Analysis of MET (soluble) (Human) by ELISA (CAT#: STEM-MB-1050-LGZ)
Analysis of MCP-5/CCL12 (Human) by ELISA (CAT#: STEM-MB-1042-LGZ)