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Analysis of IL-6 (Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0356-LGZ)

Introduction

Interleukin-6 is a cytokine of the chemokine family. IL-6 is synthesized by a variety of cells, including activated T and B cells, monocyte-macrophages, endothelial cells, epithelial cells, and fibroblasts. There are many target cells that IL-6 acts on, including macrophages, liver cells, resting T cells, activated B cells, and plasma cells; its biological effects are also very complex, and it was once called B cell stimulating factor 2 (bsf-2 ), 26KD protein, B cell differentiation factor (bCDf), hepatocyte stimulating factor (hsf), etc.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Mouse Monoclonal
Detection Antibody: Goat Polyclonal
Recombinant Standards: Expressed in Insect cell line

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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