Analysis of IL-8 (HA) (Human) by ELISA (CAT#: STEM-MB-1002-LGZ)

Introduction

The protein encoded by the IL-8 (HA) gene is a member of the CXC chemokine family. This chemokine is one of the main mediators of the inflammatory response. This chemokine is secreted by several types of cells. It acts as a chemoattractant and is a potent angiogenic factor. The gene is thought to play a role in the pathogenesis of bronchiolitis, a common respiratory disease caused by viral infection. This gene and 10 other members of the CXC chemokine gene family form a chemokine gene cluster in a region that maps to chromosome 4q.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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