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Analysis of IL-8 (HA) (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0364-LGZ)

Introduction

The protein encoded by the IL-8 (HA) gene is a member of the CXC chemokine family. This chemokine is one of the main mediators of the inflammatory response. This chemokine is secreted by several types of cells. It acts as a chemoattractant and is a potent angiogenic factor. The gene is thought to play a role in the pathogenesis of bronchiolitis, a common respiratory disease caused by viral infection. This gene and 10 other members of the CXC chemokine gene family form a chemokine gene cluster in a region that maps to chromosome 4q.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Serum, Plasma, Urine, Cell Culture Supernatant
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