Analysis of IL11RA Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2042-LGZ)

Introduction

Official Full Name: interleukin 11 receptor subunit alpha
Also known as: CRSDA
Interleukin 11 is a stromal cell-derived cytokine belonging to the family of pleiotropic and redundant cytokines that employs the gp130 transducing subunit in its high-affinity receptor. This gene encodes the IL-11 receptor, which is a member of the hematopoietic cytokine receptor family. This particular receptor is very similar to ciliary neurotrophic factor in that both contain a 2-domain extracellular region consisting of an immunoglobulin-like domain and a cytokine-like receptor domain. Multiple alternatively spliced transcript variants have been found for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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