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Analysis of IL1RL1/ST2 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0322-LGZ)

Introduction

As a new biomarker, ST2 has been widely used in recent years and is one of the main directions of future development. ST2, a protein biomarker of cardiac stress encoded by the IL1RL1 gene, is a member of the interleukin-1 receptor family, expressed as transmembrane (ST2L) and soluble isotype (sST2) subtypes. sST2 has been shown to be physiologically associated with cardiac hypertrophy, fibrosis, and ventricular dysfunction.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiac Biomarkers, Cardiac Injury, Cardiovascular, Toxicology

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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