Analysis of Infectious Hematopoietic Necrosis Virus (IHNV) by Real-Time PCR Method (CAT#: STEM-MB-3681-LGZ)

Introduction

Infectious hematopoietic necrosis virus (IHNV), a member of the genus Novirhabdovirus and family Rhabdoviridae, causes infectious hematopoietic necrosis in fish. The virus has the typical bullet shape of members of the Rhabdoviridae, and the genome consists of a single molecule of negative-sense ssRNA with varying strain pathogenicity.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.