Analysis of Infectious Pancreatic Necrosis Virus (IPNV) by Real-Time PCR Method (CAT#: STEM-MB-3685-LGZ)

Introduction

Infectious pancreatic necrosis virus (IPNV) is the causative agent of an acute and highly contagious disease of juvenile salmonids, typically at time of the commencement of feeding. IPNV is the type species of the genus Aquabirnavirus within the family Birnaviridae.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.