Analysis of Infectious Salmon Anaemia Virus (ISAV) by Real-Time PCR Method (CAT#: STEM-MB-3734-LGZ)

Introduction

Infectious salmon anemia virus (ISAV) is the causative agent of infectious salmon anemia (ISA), a disease of farmed Atlantic salmon (Salmo salar) recorded for the first time in Norway in 1984. ISAV is the type species of the genus Isavirus in the Orthomyxoviridae. The virus is adapted to cold-water salmonid fish and has an optimum growth at 15 °C.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.