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Analysis of interaction between carbon nanotubes (CNTs) and bovine serum albumin (BSA) by UV-Vis Spectroscopy (CAT#: STEM-MB-0983-WXH)

Introduction

A carbon nanotube (CNT) is a tube made of carbon with diameters typically measured in nanometers. Researchers showed that bioelectrochemical reactions can be driven by attaching proteins onto the surface of CNTs and that well-controlled aligned CNTs can be applied as immobilization matrices and as mediators for the development of biosensor devices . Not just this, researchers also focus on how to form stable colloidal dispersions in various solvents including physiological solutions, in which solubility of CNTs is extremely small. Such soluble CNTs were expected to be promising as the basis for potential biotechnological and medical applications, in particular, utilized for DNA, proteins, and drug delivery.




Principle

UV-Vis spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light that are absorbed by or transmitted through a sample in comparison to a reference or blank sample. This property is influenced by the sample composition, potentially providing information on what is in the sample and at what concentration. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time.

Applications

UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of diverse analytes or sample, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules. Spectroscopic analysis is commonly carried out in solutions but solids and gases may also be studied.

Procedure

1. Calibrate the Spectrometer
2. Perform an Absorbance Spectrum
3. Kinetics Experiments with UV-Vis Spectroscopy

Materials

UV/VIS Spectrophotometer
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