Analysis of INTS11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1345-LGZ)

Introduction

Official Full Name: integrator complex subunit 11<br />Also known as: RC68; INT11; PSF3L; RC-68; CPSF3L; CPSF73L<br />The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit and mediates the 3-prime end processing of small nuclear RNAs U1 and U2. INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Avian Adenovirus by Real-Time PCR Method (CAT#: STEM-MB-2868-LGZ)
Analysis of PRAMEF15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1382-LGZ)
Analysis of Lawsonia Spp. by Real-Time PCR Method (CAT#: STEM-MB-2942-LGZ)
Analysis of OR2M2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0521-LGZ)
Analysis of DNAJB2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1516-LGZ)
Analysis of UCK2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0738-LGZ)
Analysis of CDKN2B-AS1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1949-LGZ)
Analysis of LCE1D Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1120-LGZ)