Analysis of INTS11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1345-LGZ)

Introduction

Official Full Name: integrator complex subunit 11
Also known as: RC68; INT11; PSF3L; RC-68; CPSF3L; CPSF73L
The Integrator complex contains at least 12 subunits and associates with the C-terminal domain of RNA polymerase II large subunit and mediates the 3-prime end processing of small nuclear RNAs U1 and U2. INTS11, or CPSF3L, is the catalytic subunit of the Integrator complex.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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