Analysis of INTS3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1063-LGZ)

Introduction

Official Full Name: integrator complex subunit 3
Also known as: INT3; SOSSA; SOSS-A; C1orf60; C1orf193
The protein encoded by this gene can form a complex with human single-strand DNA binding proteins 1 or 2 (hSSB1 and hSSB2) and other proteins to mediate genome stability and the DNA damage response. The encoded protein is also part of a multiprotein complex that interacts with the C-terminal domain of RNA polymerase II large subunit to help regulate processing of U1 and U2 small nuclear RNAs.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of TFR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1850-LGZ)
Analysis of SPMIP3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0242-LGZ)
Analysis of Simian Immunodeficiency Virus (SIV) by Real-Time PCR Method (CAT#: STEM-MB-3062-LGZ)
Analysis of IL20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1069-LGZ)
Analysis of Gasterophilus Spp. by Real-Time PCR Method (CAT#: STEM-MB-3566-LGZ)
Analysis of HNRNPU Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1043-LGZ)
Analysis of OR5P3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2376-LGZ)
Analysis of Campylobacter Sputorum by Real-Time PCR Method (CAT#: STEM-MB-2707-LGZ)