Analysis of IPO7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2262-LGZ)

Introduction

Official Full Name: importin 7
Also known as: Imp7; RANBP7
The importin-α/β complex and GTPase Ran mediate nuclear import of proteins through canonical nuclear localization signals. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif associated with the Ran binding site of importin-β. Similar to importin-beta, this protein prevents RanGAP1 from activating Ran's GTPase, inhibits nucleotide exchange on RanGTP, and directly binds to the nuclear pore complex, competing with importin-beta and transport proteins for binding sites. The protein has a Ran-dependent translocation cycle that rapidly crosses the nuclear membrane in both directions. At least four importin β-like transport receptors directly bind and import ribosomal proteins, namely importin β itself, transporter, RanBP5 and RanBP7.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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