Analysis of IVL Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1058-LGZ)

Introduction

Official Full Name: involucrin<br />Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of BMPR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1508-LGZ)
Analysis of RAD54L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0106-LGZ)
Analysis of WNT3A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0753-LGZ)
Analysis of Mycobacterium Xenopi by Real-Time PCR Method (CAT#: STEM-MB-2933-LGZ)
Analysis of POLR2L Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2116-LGZ)
Analysis of ECEL1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1765-LGZ)
Analysis of RIT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0615-LGZ)
Analysis of OTOG Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2413-LGZ)