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Analysis of JNK (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0377-LGZ)

Introduction

JNK, (c-Jun N-terminal kinase), also known as stress-activated protein kinase (SAPK), is another subclass of MAPK (mitogen-activated protein kinase) signaling pathway in mammalian cells. It plays an important role in various physiological and pathological processes such as cell cycle, reproduction, apoptosis and cell stress.




Principle

Detection Technique: MSD Hypersensitive Multi-Factor Electrochemiluminescence
Capture Antibody: Rabbit Polyclonal
Detection Antibody: Mouse Monoclonal

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Whole Cell Lysate
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