Analysis of JUP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2483-LGZ)

Introduction

Official Full Name: junction plakoglobin
Also known as: PG; DP3; PDGB; PKGB; CTNNG; DPIII
This gene encodes a major cytoplasmic protein that is the only known common component in submembrane plaques of desmosomes and intermediate junctions. This protein forms a unique complex with cadherin and desmoglein-cadherin and is a member of the catenin family because it contains a unique repeating amino acid motif called the armadillo repeat. Mutations in this gene are associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully characterized.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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