Analysis of KCND3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1148-LGZ)

Introduction

Official Full Name: potassium voltage-gated channel subfamily D member 3
Also known as: KV4.3; SCA19; SCA22; BRGDA9; KCND3L; KCND3S; KSHIVB
Voltage-gated potassium channels are the most complex class of voltage-gated ion channels from both functional and structural points of view. Their diverse functions include regulation of neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes—shaker, shaw, shab, and shall—were found in Drosophila and each had human orthologs. This gene encodes a member of the potassium channel, voltage-gated, Shal-associated subfamily, members of which form voltage-activated A-type potassium channels and are prominent during the repolarization phase of the action potential. This member includes two isoforms of different sizes, which are encoded by alternatively spliced transcript variants of this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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