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Analysis of KCNJ11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2392-LGZ)

Introduction

Official Full Name: potassium inwardly rectifying channel subfamily J member 11<br />Also known as: BIR; HHF2; PHHI; IKATP; PNDM2; TNDM3; KIR6.2; MODY13<br />Potassium channels are present in most mammalian cells and they are involved in a wide range of physiological responses. The protein encoded by this gene is an integral membrane protein and inwardly rectifying potassium channel. This encoded protein, which favors the flow of potassium into cells rather than out, is controlled by the G protein and was found to be associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive disorder characterized by unregulated insulin secretion. Defects in this gene may also lead to autosomal dominant non-insulin-dependent diabetes mellitus type 2 (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). Multiple alternatively spliced transcript variants encoding different protein isoforms have been described for this gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements