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Analysis of KCNQ1OT1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2255-LGZ)

Introduction

Official Full Name: KCNQ1 opposite strand/antisense transcript 1<br />Also known as: LIT1; Kncq1; KvDMR1; KCNQ10T1; KCNQ1-AS2; KvLQT1-AS; NCRNA00012<br />Human chromosomal region 11p15.5 contains two clusters of epigenetically regulated genes expressed in a parental origin from only one chromosome. Each cluster or imprinted domain is regulated by a functionally independent imprinted control region (ICR). The human CDKN1C/kcnq10t1 domain is regulated by ICRs located in the KCNQ1 intron and comprises at least eight genes that are exclusively or preferentially expressed by maternally inherited alleles. ICR DNA is specifically methylated on maternally inherited chromosomes and specifically unmethylated on paternally inherited chromosomes. The ICR contains the promoter of the kcnq10t1 gene, which is expressed exclusively by the paternal allele. The kcnq10t1 transcript is an antisense transcript of the KCNQ1 gene, an unspliced long non-coding RNA. It interacts with chromatin and regulates the transcription of multiple target genes through epigenetic modifications. This transcript is abnormally expressed on both chromosomes in most patients with Beckwith-Wiedemann syndrome, and this transcript also plays an important role in colorectal carcinogenesis.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements