Analysis of Kyasanur Forest Disease Virus (KFDV) by Real-Time PCR Method (CAT#: STEM-MB-3754-LGZ)

Introduction

The Kyasanur Forest disease virus (KFDV) is a highly pathogenic member of the family Flaviviridae causing a zoonosis, KFD, that is transmitted by the bite of infective ticks (Haemaphysalis spinigera) primarily in its nymphal stage, and characterized by acute febrile illness with severe hemorrhagic manifestations.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.