Analysis of L-Lactic Acid (LA) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1319-LGZ)

Introduction

Sensitivity: 0.14 mmol/L
Detection range: 0.14-7.0 mmol/L
Precision: inter-lot difference of 2%, intra-lot difference of 1.6%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 530 nm

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Principle Applications Procedure Materials

Principle

Using oxidizing coenzyme Ⅰ (NAD+) as hydrogen receptor, LDH catalyzes lactate dehydrogenation to produce pyruvate, which converts NAD+ into NADH. Nitrotetrazole blue (NBT) chloride was reduced to purple formazan by hydrogen delivery by n-methylphenazine methyl sulfate (PMS), and its color depth was linearly related to the content of lactic acid.

Applications

Suitable for detecting lactic acid content in whole blood.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: Whole Blood Samples

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