Analysis of LAMB3 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1098-LGZ)

Introduction

Official Full Name: laminin subunit beta 3<br />Also known as: AI1A; LAM5; JEB1A; JEB1B; LAMNB1; BM600-125KDA<br />The product encoded by this gene is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Raillietina Cesticillus by Real-Time PCR Method (CAT#: STEM-MB-4049-LGZ)
Analysis of Parechovirus (PeV) by Real-Time PCR Method (CAT#: STEM-MB-3418-LGZ)
Analysis of NBPF11 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0393-LGZ)
Analysis of Mycoplasma Bovis by Real-Time PCR Method (CAT#: STEM-MB-2885-LGZ)
Analysis of RPS20 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1869-LGZ)
Analysis of XRCC2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1848-LGZ)
Analysis of Rhipicephalus Spp. by Real-Time PCR Method (CAT#: STEM-MB-3178-LGZ)
Analysis of LRRC26 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1986-LGZ)