Analysis of Langat Virus by Real-Time PCR Method (CAT#: STEM-MB-3603-LGZ)

Introduction

Langat virus (LGTV) was isolated in Malaysia and neighboring Thailand from pools of Ixodes granulatus and Haemaphysalis ticks. The virus infects rodents, particularly ground rats and the noisy rat or long-tailed rat.
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.