Analysis of LBR Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1094-LGZ)

Introduction

Official Full Name: lamin B receptor
Also known as: PHA; C14SR; LMN2R; PHASK; TDRD18; DHCR14B
The protein encoded by this gene belongs to the ERG4/ERG24 family. It localized in the nuclear envelope inner membrane and anchors the lamina and the heterochromatin to the membrane. It may mediate interaction between chromatin and lamin B. Mutations of this gene has been associated with autosomal recessive HEM/Greenberg skeletal dysplasia. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of DSTYK Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0912-LGZ)
Analysis of Mycoplasma Arginini by Real-Time PCR Method (CAT#: STEM-MB-3739-LGZ)
Analysis of LOC126806583 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1615-LGZ)
Analysis of Haemophilus Paragallinarum by Real-Time PCR Method (CAT#: STEM-MB-3002-LGZ)
Analysis of EFCAB2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0934-LGZ)
Analysis of Fructus Tsaoko by Real-Time PCR Method (CAT#: STEM-MB-3989-LGZ)
Analysis of TSHB Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1168-LGZ)
Analysis of LCE4A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0421-LGZ)