Analysis of Lentinus edodes-Derived Material by Real-Time PCR Method (CAT#: STEM-MB-2876-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.
Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.

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Principle Applications Procedure Materials Notes Related Products

Principle

Modern food processing technology has greatly changed the original taste, smell, color, texture and texture of food materials, so the traditional sensory identification technology has been unable to accurately identify the authenticity of food materials. At the same time, some ingredients are sensitized. Therefore, rapid and sensitive food source detection technology based on genetic detection will provide an important guarantee for food supervision and safety.
In this experiment, primers and probes were designed according to the conservative region of mushroom, which could detect the mushroom components in the sample specifically, but could not detect other non-mushroom components. The test is fast, and the entire test process (per sample) takes only 2.0 hours. The lower limit of detection of lentinus edodes in mixed samples was 0.01%, and the lower limit of nucleic acid detection of lentinus edodes in mixed samples was 0.1ng/μL.

Applications

Used to detect the presence of lentinus edodes-derived material in food or other materials.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: DNA

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