Analysis of Leucine Aminopeptidase (LAP) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1431-LGZ)

Introduction

Sensitivity: 5.2 U/L
Detection range: 5.2-201.8 U/L
Precision: inter-lot difference of 6.4%, intra-lot difference of 4.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 405 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

LAP can catalyze the substrate L-leucine-4-nitroaniline to produce p-nitroaniline, which has the maximum absorption peak at wavelength 405 nm. The enzyme activity of LAP can be calculated by measuring the increase in absorbance of the system.

Applications

Used to detect leucine aminopeptidase activity in animal tissue samples and serum (plasma) samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissue samples and serum (plasma) samples

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