Analysis of LOC110121236 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1586-LGZ)

Introduction

Gene description: VISTA enhancer hs1968<br />Gene type: biological region<br />This locus represents a conserved genomic element that may act as an enhancer. It activates the Hsp68 promoter coupled to the LacZ reporter gene in the dorsal root ganglia of transgenic mouse embryos. A subregion of the H3K27ac and H3K4me1 histone modification marks was also shown to be an active enhancer in immature human embryonic stem cells by ChIP-STARR-seq massively parallel reporter assay (MPRA). This locus also includes an accessible chromatin subregion that was validated as an enhancer based on its ability to activate an origin of replication minimal core promoter by the ATAC-STARR-seq (assay for transposase-accessible chromatin with self-transcribing act ive regulatory region sequencing) MPRA in GM12878 lymphoblastoid cells.