Analysis of Low-Density Lipoprotein Cholesterol (LDL-C) by Enzyme-labeled Instrument (CAT#: STEM-MB-1377-LGZ)

HDL, CM, VLDL and other lipoproteins (except LDL) first change their structure and dissociate under the action of surfactants. The released particulate cholesterol molecules react with cholesterol enzyme reagents, and the produced hydrogen peroxide is consumed without color in the absence of coupling agent. At this time, LDL particles are still intact, and then reagents containing coupling agent are added. It can make LDL particles dissociate and release cholesterol, cholesterol in cholesterol esterase (CE), cholesterol oxidase (CO) catalyzed, hydrogen peroxide, hydrogen peroxide in the presence of 4-amino-antipyrine (4-AA) and phenol (T-OOS), through the oxidation enzyme (POD) catalyzed, the reaction produces a red quinone compound called phenazone, whose color is proportional to the amount of LDL-C as cholesterol molecules from other lipoproteins have been removed.

It is suitable for the determination of low density lipoprotein cholesterol in serum (plasma), tissue and cell samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma), tissue and cell samples