Analysis of LRPPRC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1733-LGZ)

Introduction

Official Full Name: leucine rich pentatricopeptide repeat containing
This gene encodes a leucine-rich protein with multiple pentapeptide repeats (PPRs). The exact role of the protein is unknown, but studies suggest that it may play a role in cytoskeletal organization, vesicle trafficking, or transcriptional regulation of nuclear and mitochondrial genes. The protein is primarily localized to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene have been associated with Leye syndrome in French Canadians.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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