Analysis of LSP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2275-LGZ)

Introduction

Official Full Name: lymphocyte specific protein 1<br />Also known as: WP34; pp52<br />This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PPFIA4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0366-LGZ)
Analysis of KIF1B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0076-LGZ)
Analysis of RORC Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0642-LGZ)
High-Resolution Melt (HRM) PCR (CAT#: STEM-MB-0205-WXH)
Analysis of GDF2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2078-LGZ)
Analysis of GPR137B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1005-LGZ)
Analysis of NUP133 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0211-LGZ)
Analysis of MIR9-1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0463-LGZ)