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Analysis of LYVE-1/XLKD1 by ELISA (CAT#: STEM-MB-1538-LGZ)

Introduction

Hyaluronic acid receptor-1 (LYVE-1), as a specific marker of lymphatic endothelium, is specifically expressed in lymphatic endothelium cells and evenly distributed in the basal surface and cell surface of lymphatic endothelium cells, which is widely used to promote the research of tumor lymphatic metastasis. In recent years, the biological function of LYVE-1 itself and its role in the field of tumor metastasis have become one of the research hotspots of tumor metastasis. To elucidate the mechanism of LYVE-1 in tumor lymphatic metastasis is of great significance for establishing a new method of tumor therapy.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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