Analysis of MAPKAPK2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0403-LGZ)

Introduction

Official Full Name: MAPK activated protein kinase 2
Also known as: MK2; MK-2; MAPKAP-K2
This gene encodes a member of the serine/threonine protein kinase family. This kinase is regulated by direct phosphorylation of p38 MAP kinase. Along with p38 MAP kinase, this kinase is involved in many cellular processes, including stress and inflammatory responses, nuclear export, regulation of gene expression, and cell proliferation. Heat shock protein HSP27 is one of the substrates of this kinase in vivo. Two transcript variants of this gene have been found encoding two different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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