Analysis of MASP2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1253-LGZ)

Introduction

Official Full Name: MBL associated serine protease 2
Also known as: sMAP; MAP-2; MAP19; MASP-2; MASP1P1
This gene encodes a member of the peptidase S1 family of serine proteases. The encoded preproprotein undergoes proteolytic processing to generate A and B chains, which heterodimerize to form the mature protease. In the lectin pathway of the complement system, this protease cleaves complement components C2 and C4 to produce C3 convertase. The encoded protease also plays a role in the coagulation cascade by cleaving prothrombin to form thrombin. Patients with myocardial infarction and acute stroke exhibit reduced concentrations of the encoded protein in serum. Alternative splicing results in multiple transcript variants, at least one of which encodes the proteolytically processed isoform.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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