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Analysis of MCP-2 (Non-human primate) by ELISA (CAT#: STEM-MB-1037-LGZ)

Introduction

Monocyte chemotactic protein 2(MCP-2; CCL8) is a protein encoded by the CCL8 gene. It is a small cytokine belonging to the CC chemokine family. MCP-2 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature MCP-2 containing 75 amino acids. Its gene is encoded by three exons, and it chemotaxis and activates many different immune cells, including mast cells, eosinophils and basophils (involved in allergic reactions), as well as monocytes, T cells and NK cells involved in inflammatory responses.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Cytokines & Chemokines, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Amniotic fluid, plasma, serum, supernatant
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